Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol
نویسندگان
چکیده
منابع مشابه
Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol.
A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was ch...
متن کاملFree 3-methoxy-4-hydroxyphenylglycol determined in plasma by liquid chromatography with coulometric detection.
We describe a simple "high-performance" liquid-chromatographic method for determining free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma, with coulometric detection and with 4-methoxy-3-hydroxyphenylglycol (iso-MHPG) as the internal standard. MHPG and iso-MHPG are extracted from plasma into ethyl acetate and the extract is washed with a sodium bicarbonate solution, evaporated, reconstituted,...
متن کاملDetermination of unconjugated 3-methoxy-4-hydroxyphenylglycol by liquid chromatography for monitoring inhibition of monoamine oxidase activity in plasma.
We describe a simple method for extracting 3-methoxy-4-hydroxyphenylglycol (MHPG) from plasma. The sample is applied to a 1-mL "Extrelut" column (EM Industries) and eluted 15 min later with ethyl acetate. After mixing the eluate with pentane and back-extraction into water, we inject 25 or 50 microL of the aqueous phase onto a Beckman 15 cm x 4.6 mm (i.d.) column packed with 5-micron ODS particl...
متن کاملSerum levels of brain-derived neurotrophic factor (BDNF), proBDNF and plasma 3-methoxy-4-hydroxyphenylglycol levels in chronic schizophrenia
BACKGROUND We investigated the serum levels of brain-derived neurotrophic factor (BDNF), proBDNF and plasma 3-methoxy-4-hydroxyphenylglycol (MHPG) levels in patients with chronic schizophrenia. METHODS Sixty-eight patients who met the DSM-IV-TR criteria and had a chronic schizophrenia (CS) duration of ≥ 5 years were enrolled. Their serum brain-derived BDNF and proBDNF levels were measured by ...
متن کاملAn improved spectrophotometric method for determination of total 3-methoxy-4-hydroxyphenylglycol in urine.
A clinically useful spectrophotometric method for the determination of 3-methoxy-4hydroxyphenyiglycol (MHPC) is presented. After treatment with barium chloride, the urine is hydrolyzed with Glusulase ($-glucuronidase and sulfatase).f The hydrolysate is passed through a column of Amberlite CG-4Bt to remove any 3methoxy-4-hydroxymandelic acid (MHMA) and the pH is adjusted to neutral. The MHPG is ...
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ژورنال
عنوان ژورنال: Journal of Chromatography B: Biomedical Sciences and Applications
سال: 1986
ISSN: 0378-4347
DOI: 10.1016/s0378-4347(00)80764-1